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相关概念视频

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Translational Regulation01:29

Translational Regulation

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Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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相关实验视频

Updated: Jul 18, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

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环境响应性二元结合并稳定双链RNA.

Niall M McLoughlin1,2, Marvin A Albers1,2, Estel Collado Camps3

  • 1Department of Chemistry and Pharmaceutical Sciences, VU University Amsterdam, De Boelelaan 1108, 1081 HZ, Amsterdam, The Netherlands.

Angewandte Chemie (International ed. in English)
|August 21, 2023
PubMed
概括

双重链RNA (dsRNA) 的稳定和保护,增强细胞吸收. 这些为环境触发释放提供了一个有希望的策略,推进dSRNA治疗方法,如短干扰RNA (siRNA).

关键词:
配送RNA 配送RNA 提供RNA在RNA稳定过程中.这是一个RNA/化合物复合体.可逆度量化是一种可逆度量化.这就是TAV2b.

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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
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科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 生物医学工程 生物医学工程

背景情况:

  • 双链RNA (dsRNA) 显示出生物医学应用的重大前景.
  • 由于dsrna的稳定性和细胞吸收不佳,dsrna的治疗用途受到阻碍.
  • 现有的dsRNA稳定策略缺乏广泛的适用性.

研究的目的:

  • 设计和评估用于dsrna稳定和传递的二元接.
  • 调查这些的保护dsrna免受降解的能力.
  • 评估dsrna的环境触发释放的可能性.

主要方法:

  • 基于RNA结合蛋白TAV2b的二元合的设计.
  • 通过二硫化键形成二元体,以模仿自然组装.
  • 评估dsrna结合,血清稳定,细胞吸收和在减少条件下释放.

主要成果:

  • 二元有效地结合和稳定血清中的dsRNA,防止降解.
  • 结合增强了dsrna的细胞吸收.
  • 在还原条件下,二元分解成单体,导致RNA结合的丧失.

结论:

  • 基于的RNA结合剂是dsRNA稳定和保护的可行策略.
  • 双相聚合提供了一种环境触发RNA释放的方法.
  • 这种方法可以扩大dsRNA的治疗应用,包括siRNAs.