小型非编码RNA B11调节了Mycobacterium abscessus毒性的多个方面
Michal Bar-Oz1, Maria Carla Martini2, Maria Natalia Alonso2
1Koret School of Veterinary Medicine, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
PLoS pathogens
|August 21, 2023
概括
小调节性RNA B11控制着Mycobacterium abscessus中的基因表达和毒性. B11中的突变与临床菌株中毒性和抗生素耐药性增加有关.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 菌根是一种重要的病原体,特别是在囊性纤维化患者中.
- 小调节性RNAs (sRNAs) 在M. abscessus基因调节中的作用在很大程度上仍未被探索.
研究的目的:
- 为了研究sRNA B11在M. abscessus基因调节和毒性中的功能.
- 确定B11对病原体表型和抗生素耐药性的影响.
主要方法:
- 在M. abscessus中,B11sRNA的删除突变发生.
- 使用RNA测序 (RNAseq) 和蛋白质学对基因表达的分析.
- 在突变菌株和临床隔离物中评估毒性和抗生素耐药性.
主要成果:
- 删除B11导致了粗略的殖民地形态,增加了促炎信号,增强了感染模型中的毒性,以及更大的抗生素耐药性.
- 在B11删除突变体中,超过200个基因被差异地表达.
- 具有B11突变的临床分离体显示了类似的表达趋势,表明功能部分丧失.
- B11通过与目标基因的核糖体结合部位 (RBS) 结合直接抑制转化,包括ESX-4分泌系统的组成部分.
结论:
- 在M. abscessus中,B11充当直接的负调节器,并可能是基因表达的间接的正调节器.
- B11对基因表达和临床相关的表型产生类效应.
- 临床菌株的低形态B11突变表明,在某些临床条件下,M. abscessus可能具有优势.
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