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上呼吸道感染模型OC43用于研究气道免疫修饰疗法

Jason L N Girkin¹, Nathan E Bryant¹, Su-Ling Loo¹

¹Hunter Medical Research Institute, University of Newcastle, Newcastle, New South Wales, Australia.

American journal of respiratory cell and molecular biology

|August 21, 2023

概括

在上呼吸道 (URT) 中使用人类冠状病毒OC43的小鼠模型有效地证明了鼻腔抗病毒免疫力. 针对托尔类受体2 (TLR2) 的治疗方法在治疗URT感染方面表现有前途.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
呼吸系统医学呼吸系统医学

背景情况:

上呼吸道 (URT) 感染,包括像OC43这样的冠状病毒引起的感染,是严重的公共卫生问题.
鼻腔粘膜中有效的抗病毒免疫反应对于预防严重疾病和传播至关重要.
缺乏合适的URT感染模型阻碍了对冠状病毒免疫的鼻腔治疗的开发.

研究的目的:

在URT中建立和验证OC43感染的小鼠模型.
在这个模型中评估针对鼻腔的免疫修饰治疗的疗效.
调查托尔样受体2 (TLR2) 在β冠状病毒先天免疫力中的作用.

主要方法:

在野生型Balb/c小鼠中进行OC43的鼻腔内接种.
在呼吸道中评估病毒热带和复制.
抗病毒细胞因子 (例如,IFN-λ) 的量化和基因表达分析.
评估免疫调节疗法:TLR2/6激动剂 (INNA-X) 和皮质类固醇 (酸).

主要成果:

OC43在小鼠鼻上得到了有效复制,病毒RNA在感染后2天达到峰值.
预防性INNA-X治疗增强了病毒诱导的IFN-λ和IFN刺激的基因表达.
鼻内注射的酸增加了病毒载量和抑制了IFN反应,这种效应在之前的INNA-X治疗中被部分减轻.

结论:

鼠标鼻上皮是易受OC43感染的,为URT冠状病毒研究建立了相关模型.
TLR2激活是对抗贝塔冠状病毒的先天抗病毒免疫力的关键决定因素.
鼻腔免疫调节疗法,特别是TLR2激动剂,对于URT冠状病毒感染具有治疗潜力.

关键词:

在 OC4343 的位置上.在TLR2中,它是TLR2.冠状病毒冠状病毒的新冠病毒.这是先天免疫力.鼠标模型鼠标模型鼠标模型

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