相关实验视频

Updated: Jul 18, 2025

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Published on: January 1, 2016

在长期的膜生物反应器运行中,是否对微生物群体动态产生影响?

Kibaek Lee¹, Yeong-Jun Park², Tahir Iqbal³

¹Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea; Advanced Institute of Water Industry, Kyungpook National University, Daegu 41566, Republic of Korea.

|August 21, 2023

概括

质量灭 (QQ) 有效地减少了膜生物反应器 (MBR) 的生物污染,而不会改变微生物群落结构. QQ影响了生物膜网络,但功能基因在很大程度上不受影响,为生物污染控制机制提供了洞察力.

科学领域:

环境微生物学环境微生物学
生物技术是生物技术.
水处理处理水的方法

背景情况:

膜生物反应器 (MBR) 中的生物污染是一个重大的运营挑战.
使用细菌菌株的定数灭 (QQ) 已经显示出缓解MBR生物污染的承诺.
在MBR中,QQ对整体微生物社区结构和功能的影响需要进一步研究.

研究的目的:

分析QQ对MBR中的微生物群体的长期影响.
了解QQ如何影响微生物社区结构,多样性和在各种操作条件下的功能.
阐明QQ影响MBR生物污染的机制.

主要方法:

在不同的空气,QQ剂量和料类型下长期 (>250天) 监测MBR微生物群落.
在混合液体和生物膜中分析微生物群体结构和多样性.
规范性对应分析和共发生网络分析,以评估社区动态.
功能性基因的遗传学研究与定数感知相关.

主要成果:

QQ治疗没有显著改变微生物社区结构或MBR中的多样性.
微生物群落主要受到料类型和操作阶段的影响.
生物膜微生物网络与真实的废水更复杂,在QQ下显示出更多的积极相互作用.
在混合液中,QQ对功能定数感应基因的影响很小,但在较高剂量的生物膜中影响了它们.

结论:

QQ是一种可行的策略,可以减少MBR生物污染,而不会破坏整个微生物群体.
料成分和操作阶段是MBR中微生物社区动态的关键驱动因素.
QQ调节生物膜结构和相互作用,有助于控制生物污染,对散装液体中的核心功能基因的影响最小.

关键词:

生物污染是一种生物污染.膜生物反应器是一个膜生物反应器.微生物社区的微生物社区.微生物网络是一个微生物网络.议员数量的灭.

更多相关视频

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

Author Spotlight: Unraveling the Mysteries of Terrestrial Anaerobic Microorganisms in Uncharted Environments by In Situ Culturing

Author Spotlight: Unraveling the Mysteries of Terrestrial Anaerobic Microorganisms in Uncharted Environments by In Situ Culturing

Published on: January 12, 2024

相关概念视频

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...

Biofilms

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...