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SARS-CoV-2 感染的严重程度和死亡率通过反复调节替代拼接的重复调节来调节
Priyanka Mehta1,2, Partha Chattopadhyay1,2, Varsha Ravi1
1Division of Immunology and Infectious Disease Biology, INtegrative GENomics of HOst-PathogEn (INGEN-HOPE) Laboratory, CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB) , Delhi, India.
Microbiology spectrum
|August 21, 2023
概括
COVID-19 感染改变了宿主基因表达和拼接. 死亡率较高的患者表现出减少的转录多样性和被重复元素改变的拼接调节,影响疾病严重程度.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 病毒学 病毒学
背景情况:
- 通过劫持宿主机器,SARS-CoV-2感染会引起各种不同的临床症状.
- 传统研究侧重于基因水平表达,忽视了转录水平的变化和拼接失调.
- 替代拼接在宿主对病毒感染的反应中起着至关重要的作用.
研究的目的:
- 为了调查COVID-19患者在严重程度亚现象类型中的转录组差异.
- 了解替代拼接在COVID-19疾病严重程度和临床结果中的作用.
- 阐明潜在的基因组调节机制,驱动SARS-CoV-2感染中的替代拼接.
主要方法:
- 125名住院COVID-19患者的宿主转录组测序.
- 转录级差异表达分析和差异异异型使用调查.
- 在差异表达转录 (DET) 和基因组调控特征中的拼接模式的分析.
主要成果:
- COVID-19死亡率患者表现出减少的转录长度和多样性.
- 在差异表达的蛋白质编码转录中观察到改变了促进体位的使用.
- 在拼接地点附近的重复 (SINE/Alu) 丰富表明重复介导的替代拼接调节.
结论:
- 替代拼接是COVID-19疾病严重程度的显著,但未被认可的调节器.
- 转录组失调,包括改变的拼接,有助于COVID-19的各种临床结果.
- 重复元素可能在调节替代拼接和影响SARS-CoV-2感染严重程度方面发挥作用.
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