三轴生物打印具有营养道的大型血管化结构
Junbiao Zhang1,2, Srisurang Suttapreyasri3, Chidchanok Leethanakul1
1Orthodontic Section, Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Songkhla, Thailand.
Biomedical materials (Bristol, England)
|August 21, 2023
概括
这项研究引入了一种新的三轴3D生物打印技术,用于制造大规模的血管化组织. 该方法精确地加载多种细胞类型并形成营养道,克服了组织工程中的关键挑战.
科学领域:
- 生物材料工程 生物材料工程
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 生物打印为组织和器官再生提供了显著的潜力.
- 生物打印大规模组织的一个主要障碍是精确共载多种细胞类型,同时保持必要的营养物质运输道.
研究的目的:
- 开发和验证一种新的3D生物打印策略,用于制造大规模的血管化组织.
- 精确封装多种细胞类型,同时在工程组织中创建功能营养道.
主要方法:
- 采用三轴3D生物打印方法,使用由凝甲基酸盐 (GelMA) 和藻酸盐 (Alg) 组成的生物墨水.
- 人类骨髓介质干细胞和人静脉内皮细胞被装入生物油墨层,而化 (CaCl2) 溶液形成了内部通道.
- 这些结构经过光聚合和离子交联,以确保结构稳定性和通道完整性.
主要成果:
- 一种GelMA/Alg生物墨水混合物 (5% w/v GelMA,1% w/v Alg) 在室温下表现出极好的打印能力和 perfusion 能力.
- 与非细胞结构相比,具有集成营养道的结构证明了细胞存活,增殖,扩散,迁移和血管网络形成的增强.
- 虽然通道包含影响了机械性能 (压缩模量较低,膨胀较高),但它并没有显著改变降解配置.
结论:
- 三轴3D生物打印是一种可行的策略,用于制造大规模的血管化组织结构.
- 这种方法可以实现精确的多细胞封装和同时形成营养道,解决当前组织工程的关键局限性.
- 开发的技术对准备复杂,大规模的血管化组织用于再生医学应用具有前景.
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