极性扩展液态染色学-三重四极质谱法用于同时进行水友和水害代谢物分析
Rui Guo1, Qisheng Zhong2, Jiaqi Liu2
1The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
Analytica chimica acta
|August 21, 2023
概括
一种新的二维液态染色学/质谱学方法使得水友性和水性代谢产物能够同时分析. 这种先进的代谢学方法可以在各种生物样本中识别数百种代谢物,并有助于发现疾病生物标志物.
科学领域:
- 代谢学 代谢学 代谢学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 在当前的代谢分析中,同时检测水友性和疏水性代谢产物是一个重大挑战.
- 现有的方法通常需要多次分析运行或专门的样本准备技术.
- 需要采用统一的方法来全面描述代谢组.
研究的目的:
- 开发和验证在线二维液态色谱/三重四极质谱 (2D-LC/TQMS) 方法.
- 为了能够同时量化各种生物矩阵中的水友性和水害性代谢物.
- 为广泛的新陈代谢分析建立一个强大的平台.
主要方法:
- 在线水友互动液态染色学 (HILIC) 与逆相液态染色学 (RPLC) 相结合.
- 三重四极质谱 (TQMS) 用于敏感和选择性检测.
- 分析了417种具有生物意义的代谢物,其logP值范围广泛 (-10.3至21.9).
主要成果:
- 2D-LC/TQMS方法成功测量了417种参与关键代谢途径的代谢物.
- 证明了优秀的方法性能,具有良好的可重现性 (RT <2%,峰值面积 <30%),线性 (R2 >0.9),以及广泛的线性范围 (0.00255μg/mL).
- 在各种生物样本 (血,血清,尿液,便,精液等离子体,肝脏) 中检测到208种常见代谢物,突出显示了该方法的适用性.
结论:
- 开发的2D-LC/TQMS方法为全面的水友性和水性代谢物分析提供了强大的工具.
- 这种方法为各种生物样本的代谢学研究提供了高吞吐量和广泛的覆盖.
- 对膀癌尿样的应用确定了30种不同的代谢物,证明了其在疾病生物标志物发现中的实用性.
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