总体分析系统用于确定人体血中的尿性毒素,基于珠子注射固体相提取,并连接到质谱学
Sara R Fernandes1, Luisa Barreiros1, Benedita Sampaio-Maia2
1LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313, Porto, Portugal; Escola Superior de Saúde, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 400, 4200-072, Porto, Portugal.
Analytica chimica acta
|August 21, 2023
概括
一种新的自动化方法量化了人体血中的氧硫酸盐 (INDS) 和p-cresol硫酸盐 (pCS). 这种技术准确地测量了这些尿性毒素,对于了解慢性病进展和心血管风险至关重要.
科学领域:
- 分析化学 分析化学
- 临床化学 临床化学
- 生物化学 生物化学
背景情况:
- 内氧硫酸盐 (INDS) 和p-cresol硫酸盐 (pCS) 是关键的尿素毒素,与慢性病 (CKD) 的进展和心血管风险有关.
- 准确量化循环INDS和pCS水平对于患者管理至关重要.
研究的目的:
- 开发和验证一种自动化分析策略,用于在人体血中量化INDS和pCS.
- 建立一种快速可靠的方法来评估这些关键的尿素毒素.
主要方法:
- 一个自动在线微固体相提取 (μSPE) 程序是使用珠子注入 (BI) 概念在实验室上门 (LOV) 格式开发的.
- SPE-BI-LOV系统被连接到并联质谱 (MS/MS) 检测,消除了先前染色分离的需要.
- 阳离子交换OASIS WAX吸附剂和特定的化物组成被优化,以最大限度地回收分析物.
主要成果:
- 开发的μSPE-BI-LOV-MS方法在血中进行INDS和pCS量化时显示出极好的线性 (r2 > 0.999).
- 达到的量化限值 (LOQ) 为INDS的2微克/毫升,为pCS的7微克/毫升.
- 整个分析周期,包括样本准备和检测,在不到20分钟内完成,成功分析了人体血样本.
结论:
- 自动化μSPE-BI-LOV-MS方法提供了一种高效且准确的方法,用于量化人体血中的氧硫酸和p-cresol硫酸.
- 这种环保的方法为临床研究和与慢性病相关的诊断提供了宝贵的工具.
- 该方法的速度和准确性支持改善尿素毒素和相关健康风险的监测.
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