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与SARS-CoV-2Delta相关的G15U突变对s2m元素二元化及其与miR-1307-3p的相互作用的影响

Caylee L Cunningham¹, Caleb J Frye¹, Joseph A Makowski¹

¹Department of Chemistry and Biochemistry, Duquesne University, Pittsburgh, Pennsylvania 15282, USA.

RNA (New York, N.Y.)

|August 21, 2023

概括

这是SARS-CoV-2的Delta变种.

科学领域:

病毒学病毒学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

SARS-CoV-2 s2m RNA 结构是一个保存的治疗点.
与原来的SARS-CoV-2相比,Delta变种中的s2m元素的研究较少.
德尔塔SARS-CoV-2具有很高的传染性和疾病严重程度.

研究的目的:

识别和定义Delta和原始SARS-CoV-2之间的s2mRNA结构的变化.
研究这些变化对s2m二分化和宿主微RNA相互作用的影响.
分析G15U突变对与宿主miR-1307-3p结合的影响.

主要方法:

针对s2m元素突变的GISAID数据库的生物信息分析.
质子核磁共振 (1H NMR) 光谱法用于比较s2m结构.
评估病毒N蛋白伴侣活性和宿主微RNA结合.

主要成果:

在>99%的德尔塔SARS-CoV-2s2m序列中发现了单个核酸突变 (G15U).
通过关闭U15-A29基对,G15U突变稳定了s2m结构的上茎.
G15U突变显著影响宿主miR-1307-3p与s2mRNA的结合.

结论:

在三角洲SARS-CoV-2中G15U突变改变了s2m的二次结构.
这种改变会影响宿主miR-1307-3p的结合,可能会影响病毒免疫反应.
这些发现强调了研究变异特异性RNA结构变化的重要性.

关键词:

在SARS-CoV-2的三角洲.分化二元化是指二元化的过程.接吻复杂的吻复杂的在 miR-1307-3pp 中使用.s2mm 这样就好了.

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