雷多克斯驱动的B12-联体开关驱动的CarH光响应驱动
Harshwardhan Poddar1, Ronald Rios-Santacruz2, Derren J Heyes1
1Manchester Institute of Biotechnology, Department of Chemistry, University of Manchester, Manchester, UK.
Nature communications
|August 21, 2023
概括
CarH光受体的光反应涉及B12 Co-C键裂变,导致四聚体解离. 介质状态和希斯提丁结合变化驱动了这个过程,整合了光和氧化复原化学.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 摄影生物学 摄影生物学
背景情况:
- 卡尔是一种依赖于B12共酶的光受体,调节着胡卜素生物合成.
- 光触发的CarH四聚合物解离和转录启动的机制尚未完全理解.
研究的目的:
- 为了阐明介质状态和分子机制的基础光诱导的CarH四聚合物解离.
- 为了研究B12光化学和氧化复原化学在CarH结构变化中的作用.
主要方法:
- 在照明后,CarH B12结合域的X射线晶体学.
- 解决方案光谱学和计算研究.
- 西丁结合残留物的局部定向突变发生.
主要成果:
- 照明揭示了CarH四聚合物解离之前的不可预见的中间状态.
- Co-C 键裂解触发了科林环的重定向,以及西丁-联结的切换 (下至上).
- 这种结合开关对于单体的形成至关重要,在有氧和无氧条件下都保持稳定.
结论:
- 碳化合物光反应整合了B12的光和氧化还原化学.
- 逐步的结变化驱动大规模的结构变化,导致四聚体解离和转录调节.
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