在葡萄牙模拟气候下,对具有工业意义的外聚糖生成蓝藻进行生物勘探
José Diogo Cruz1,2, Cédric Delattre3,4, Aldo Barreiro Felpeto2
1Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007, Porto, Portugal.
Scientific reports
|August 21, 2023
概括
研究人员探索了葡萄牙的蓝菌株,以实现可持续的生物聚合物生产. 两种菌株,Synechocystis sp. 这两种菌株. 在LEGE 07367和Chroococcales 菌LEGE 19970中,显示出高产的独特,无毒素释放的多糖化物 (RPS).
科学领域:
- 生物技术和生物聚合物生产
- 微生物生理学和生态学
背景情况:
- 菌外聚糖 (EPS) 显示出作为可持续生物聚合物的前景.
- 目前,高昂的生产成本限制了蓝细菌EPS的商业利用.
- 在工业条件下识别新的EPS生产菌株对于生物技术应用至关重要.
研究的目的:
- 对葡萄牙的蓝菌株进行生物探测和特征化,用于EPS生产.
- 在模拟的葡萄牙气候条件下评估生长性能和生物质组成.
- 为了确定产生高产量的工业相关释放的多糖体 (RPS) 的菌株.
主要方法:
- 选择了25种不同的葡萄牙蓝菌菌株用于种植.
- 在模拟葡萄牙气候的日照光和温度下种植菌株.
- 评估了生长表现,近端宏观营养素组成和RPS产量.
主要成果:
- 赛内科西斯蒂斯和赛亚诺属表现出快速增长 (0.1-0.2 g L−1 day−1).
- 综合囊 sp. 的情况. LEGE 07367 和 Chroococcales 菌LEGE 19970分别产生了0.3和0.4克L-1的RPS.
- 这些RPS是基于葡萄糖的,高分子量,低单糖,无毒素.
结论:
- 确定了有前途的蓝藻细菌菌株 (Synechocystis sp. LEGE 07367, Chroococcales cyanobacterium LEGE 19970) 用于生物聚合物生产.
- 在当地气候条件下证明了蓝藻细菌EPS生物工艺的可行性.
- 这些发现是开发可持续的蓝细菌EPS生物工艺的初步步骤.
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