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Updated: Aug 10, 2026

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
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高速多平面共聚焦显微镜用于密集标记的神经元群体的电压成像

Timothy D Weber1, Maria V Moya1,2, Kıvılcım Kılıç1,3

  • 1Department of Biomedical Engineering, Boston University, Boston, MA, USA.

Nature neuroscience
|August 21, 2023
PubMed
概括

我们开发了一种用于基因编码电压指示器 (GEVIs) 的新成像技术,可以改善对比度和监测神经元活动的速度. 这种方法增强了密集的神经群体的成像,这对于理解大脑功能至关重要.

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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...