结构/表位分析和IgE结合活动来自Dermatophagoides pteronyssinussinus的三种环菲林家族蛋白质
Yuwei Li1,2,3, Xizhuo Sun2, Liteng Yang4,5
1Department of Respiratory Medicine, The Seventh Affiliated Hospital of Southern Medical University, Foshan, 528244, Guangdong, China.
Scientific reports
|August 21, 2023
概括
环素 (CyPs) 是参与细胞功能和免疫力的蛋白质. 这项研究发现,CyPE与过敏原CyPA具有结构和进化相似之处,两者都表现出IgE活性,与CyPB不同.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 环素 (CyPs) 是关键的蛋白质,涉及到基本的细胞过程和各种病理生理条件.
- 几种CyP参与调节免疫系统,可以作为疾病的病因因因子.
研究的目的:
- 分析三种Cyclophilin家族成员的物理化学和免疫学特征:CyPA,CyPB和CyPE.
- 研究CyPA,CyPB和CyPE的进化关系,结构相似性和B细胞表位样.
- 用患者血清评估CyPA,CyPB和CyPE的体内过敏性潜力.
主要方法:
- 在体免疫信息学工具被用来预测CyPE和CyPA的B细胞表位.
- 三种CyP蛋白 (CyPA,CyPE,CyPB) 被克隆并从Dermatophagoides pteronyssinus中表达出来.
- 酶相关免疫吸收试验 (ELISA) 用于评估患者血清中对表达的CyP蛋白的IgE反应性.
主要成果:
- CyPE与过敏原CyPA表现出更密切的进化关系和显著的结构/抗原相似性.
- 在分析中,发现了CyPE和CyPA共同的三个共识B细胞表位.
- 埃利萨检测结果显示IgE活性对抗CyPA (21.4%) 和CyPE (7.1%),但没有CyPB (0%).
结论:
- CyPE与已知的过敏原CyPA具有重要的结构和免疫特征,表明潜在的交叉反应性.
- 在CyPE中的RNA结合基因可能会减弱其PPIase域的免疫性.
- 在CyPB的B细胞表位上发生的结构性突变可能解释了其缺乏观察到的IgE活性.
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