血外基因组 lncRNA-miRNA-mRNA网络构建及其在第一发精神分裂症诊断疗效的识别
Xinzhe Du1,2, Jinzhi Lv3, Jianping Feng1
1Department of Psychiatry, First Hospital/First Clinical Medical College of Shanxi Medical University, Taiyuan, China.
BMC psychiatry
|August 22, 2023
概括
这项研究揭示了第一发精神分裂症 (FOS) 的外体长非编码RNA-microRNA-信使RNA网络. 这些网络显示出作为诊断精神分裂症的新生物标志物的潜力.
科学领域:
- 神经科学是一个神经科学.
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 精神分裂症 (FOS) 研究缺乏对外体调节网络的理解.
- 外体提供潜力作为精神疾病的诊断生物标志物.
研究的目的:
- 在第一发精神分裂症 (FOS) 中调查外体长非编码RNA (lncRNA) -microRNA (miRNA) -信使RNA (mRNA) 网络.
- 探索这些外体网络作为FOS的诊断生物标志物的潜力.
主要方法:
- 在10名FOS患者和10名健康对照 (HCs) 中,高通量血外体lncRNA,miRNA和mRNA的测序.
- 构建lncRNA-miRNA-mRNA网络,与PANSS分数进行相关性分析,基因本体学 (GO) 和KEGG通路分析,以及接收器操作特征 (ROC) 分析.
主要成果:
- 在FOS外体中鉴定了746个差异表达的lncRNA,22个miRNA和2637个mRNA.
- 构建了竞争的内源RNA (ceRNA) 网络,揭示了EZH2和SIRT1作为枢纽基因.
- 外体网络的诊断精度 (AUC) 比单个组件的诊断精度更高.
结论:
- 在FOS患者的外体中建立了第一个lncRNA-miRNA-mRNA网络.
- 已识别的外体调节网络具有作为FOS诊断的新生物标志物的巨大潜力.
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