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探索患有糖尿病前期和未接受过治疗的2型糖尿病患者的肠道微生物群 - 一项试点研究
Kristin Gravdal1, Katrine H Kirste2, Krzysztofa Grzelak2
1Genetic Analysis AS, Ulvenveien 80B, 0581, Oslo, Norway. kristin.gravdal@genetic-analysis.com.
改变的肠道细菌,包括SCFA生产者和机会性细菌,可以将2型糖尿病 (T2D) 和糖尿病前期患者与健康人区分开来. 需要进一步的研究来定义特定的糖尿病微生物群签名.
科学领域:
- 微生物组研究的研究.
- 代谢性疾病研究研究.
- 人体肠道微生物组分析
背景情况:
- 2型糖尿病 (T2D) 与肠道失调有关,影响胰岛素敏感性,脂质概况和炎症.
- 一个明确的T2D肠道微生物群概况仍然没有建立.
- 这项试点研究调查了与健康对照组相比,糖尿病前期和未经治疗的T2D患者的肠道微生物群.
研究的目的:
- 探索糖尿病前期和T2D患者的肠道微生物群组成.
- 确定与糖尿病前期和T2D相关的特定细菌种群.
- 为了比较糖尿病前期,T2D和健康个体之间的肠道微生物群概况.
主要方法:
- 从24名T2D前患者,18名T2D患者和52名健康的挪威人身上收集了便样本.
- 使用两个16S rRNA基因测序技术分析细菌DNA:GA-map® 131-plex和LUMI-SeqTM.
- 使用多变量分析,差异丰度估计和分类模型来识别微生物变异和歧视性特征.
主要成果:
- 通过LUMI-SeqTM,分别在T2D前/健康组和T2D/健康组之间分别有10个和9个细菌种群的差异丰富.
- 十种细菌标志物使用GA-map®分析将T2D前与健康受试者区分开来.
- 不同丰富的细菌包括短链脂肪酸 (SCFA) 生产者和机会性病原体,有助于分离群体.
结论:
- 与健康个体相比,SCFA产生和机会性细菌的变化是T2D前和T2D患者肠道微生物群的特征.
- 分类模型为T2D前和T2D组确定了不同的微生物特征.
- 需要进一步的研究,以确定特定的糖尿病微生物群签名,考虑诸如BMI这样的因素.
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