多功能可转化的生物合成,用于诱导瘤细胞亡
Yufei Di1,2, Qi Shen1, Zhiwen Yang1,2
1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
Small (Weinheim an der Bergstrasse, Germany)
|August 22, 2023
概括
工程细菌产生蛋白质纳米颗粒,转化为纳米纤维,触发癌细胞死亡. 这种新的方法通过线粒体依赖性亡提供了精确的,光控制的,体内癌症治疗.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 分子生物学分子生物学
背景情况:
- 工程纳米材料提供了增强的疾病治疗特异性.
- 从生物来源开发新型治疗剂对于有针对性的治疗至关重要.
- 基于蛋白质的材料可以设计为受控的形态变化.
研究的目的:
- 从大肠杆菌 (E. coli) 开发一种完全基于蛋白质的材料,用于癌症治疗.
- 设计一种系统,使蛋白质纳米粒子在现场形态转化为纳米纤维.
- 通过光诱导释放,创建一个空间时间可控的抗癌系统.
主要方法:
- 合成了一种基于蛋白质的材料 (P1),包括KLVFF,BAK蛋白和GFP与准部分.
- 在Cathepsin B的存在下,研究了P1纳米颗粒在现场转化为纳米纤维的过程.
- 通过涂层P1-表达大肠杆菌与阴离子结合的多电解质,构建了一个光控制系统.
主要成果:
- P1纳米粒子转化为纳米纤维,促进线粒体外膜上的BAK二元化.
- 通过Bcl-2通路通过线粒体依赖性亡在体外和体内获得有效的抗癌活性.
- 从大肠杆菌中释放出精确的,光控制的,以示现场治疗效果.
结论:
- 基于蛋白质的酶催化转化策略为向药物输送提供了一个新的视角.
- 这种生物合成方法显示了精确疾病治疗的巨大潜力.
- 具有光诱导性释放的工程大肠杆菌提供了一个空间时间可控制的抗癌系统.
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