通过工程化囊蛋白进行定量交叉链接,以研究细菌原酶中的域间相互作用

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Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

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Jamil Serwanja1, Hans Brandstetter1, Esther Schönauer1

  • 1Department of Biosciences and Medical Biology, Paris Lodron University of Salzburg, Salzburg 5020, Austria.

STAR protocols
|August 22, 2023
PubMed
概括

这项研究引入了一种新的蛋白质交叉链接方法,使用工程氨酸. 该协议有助于研究多域蛋白质的域间运动,如原酶G.

关键词:
分子生物学分子生物学蛋白质生物化学 蛋白质生物化学结构生物学 结构生物学

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