在绝经后骨质疏松症中鉴定与氧化压力相关基因相关的生物标志物
Dan Liu1, Zhijun Hu2, Zhanying Tang3
1Department of Rehabilitation Medicine, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China. danliu0212@163.com.
Cellular and molecular biology (Noisy-le-Grand, France)
|August 22, 2023
概括
这项研究确定了与氧化压力相关的基因DDIT3和FOXO3作为绝经后骨质疏松症 (PM-OP) 的潜在生物标志物. 这些发现为通过解决抗氧化剂系统缺陷来预防和治疗PM-OP提供了新的目标.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 骨质疏松症 (OP) 是一种普遍的代谢性疾病,衰老和更年期是主要的风险因素,影响了三分之一的绝经后妇女.
- 在了解预防和治疗绝经后骨质疏松症 (PM-OP) 的抗氧化剂系统方面存在重大差距.
- 氧化应激 (OS) 与各种疾病有关,但其在PM-OP中的特定作用需要进一步阐明.
研究的目的:
- 为了确定不同表达的氧化压力相关基因 (DE-OSRGs),特定于绝经后骨质疏松症 (PM-OP).
- 通过生物信息学和机器学习方法发现PM-OP的新型诊断生物标志物.
- 探索与PM-OP相关的潜在分子机制和监管网络.
主要方法:
- 差异基因表达分析 (Limma) 和加权基因共表达网络分析 (WGCNA) 用于识别DE-OSRG.
- 蛋白质与蛋白质相互作用 (PPI) 网络构建,机器学习算法 (SVM-RFE,RF) 和ROC曲线分析被用来识别特征基因.
- 进行基因组丰富分析 (GSEA) 和TF-miRNA-mRNA调控网络构建以探索机制. qRT-PCR验证的基因表达. qRT-PCR验证的基因表达.
主要成果:
- 通过交叉模块化和差异性基因确定了20个针对PM-OP的DE-OSRG.
- 确定DDIT3和FOXO3是具有PM-OP诊断潜力 (AUC>0.7) 的关键特征基因.
- GSEA揭示了特征基因在中性粒细胞激活,氧化酸化和MAPK信号通路中的参与. qRT-PCR证实了PM-OP中的FOXO3和DDIT3上调.
结论:
- DDIT3和FOXO3被确定为绝经后骨质疏松症的新型氧化压力相关生物标志物.
- 这些基因可以作为PM-OP.的临床治疗的潜在治疗点.
- 这项研究提供了有关氧化应激的PM-OP分子机制的见解.
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