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Updated: Jul 18, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

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Published on: October 15, 2019

主要开角青光眼 (POAG) 和肠道微生物群之间的相关性:对预测,预防和个性化医学的试点研究

Si Chen^1,2,3, Nan Wang^1,2,3, Siqi Xiong^1,2,3

¹Eye Center of Xiangya Hospital, Central South University, Changsha, 410008 Hunan China.

The EPMA journal

|August 22, 2023

概括

这项研究确定了六个关键基因 (NFKB1,IL18,KITLG,TLR9,FKBP2和HDAC4),这些基因将肠道微生物群与原发性开角玻璃眼 (POAG) 联系起来. 这些发现突显了巨细胞介导的免疫反应在POAG中的作用,为个性化治疗提供了潜在的生物标志物.

科学领域:

眼科医生眼科眼科
免疫学免疫学免疫学
微生物组研究微生物组研究

背景情况:

玻璃眼是全球不可逆转失明的主要原因.
新出现的证据表明,青光眼与免疫系统功能障碍有关.
肠道微生物群 (GM) 在免疫平衡中起着至关重要的作用,但其对青光眼的影响尚不清楚.

研究的目的:

调查关键分子和途径,调解转基因生物和玻璃眼之间的关系.
为了确定潜在的新生物标志物,用于预测,预防和个性化医学在绿眼病.

主要方法:

下载并分析了初级开角青光眼 (POAG) 患者和转基因相关基因的数据集.
在健康和POAG样本之间确定了差异表达基因 (DEG).
对转基因相关的DEG进行了相关性分析,通路丰富和蛋白质与蛋白质相互作用 (PPI) 网络分析.
在体外玻璃眼瘤模型中使用定量实时聚合酶链反应 (RT-qPCR) 验证的枢纽基因表达.

主要成果:

在POAG中确定了16种与转基因相关的DEG (9种上调,7种下调).
途径丰富分析揭示了免疫调节中的丰富,特别是与巨细胞相关的途径.
通过PPI网络分析确定了6个枢纽基因.
通过RT-qPCR验证了在体外玻璃眼瘤模型中枢基因的表达.

结论:

解析了六个核心基因 (NFKB1,IL18,KITLG,TLR9,FKBP2和HDAC4),这些基因对POAG和转基因调控至关重要.
突出了巨细胞介导的免疫反应在POAG中的重要作用.
这些枢纽基因代表了未来预测,预防和个性化诊断和治疗眼的潜在目标.

关键词:

生物标志物生物标志物眼光障碍眼光障碍眼光障碍眼光障碍我们的肠道微生物群.炎症炎症是一种炎症.实验室医学实验室医学巨细胞是什么?巨细胞是什么?预测性预防性个性化医学 (PPPM/3PM)

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