在中国汉族人口中,SPARC多态和结性脊柱炎及其mRNA和蛋白质表达之间的关联:一个案例对照研究
Dongxia Liu1, Liya Wang1,2, Zhongdan Hu3
1Department of Rheumatology and Immunology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, People's Republic of China.
International journal of general medicine
|August 22, 2023
概括
在分泌蛋白酸性和丰富的囊蛋白 (SPARC) 的遗传变异与结性脊髓炎 (AS) 的风险有关. 在AS患者中,SPARC mRNA和蛋白质水平显著降低,这表明它在疾病发展中的作用.
科学领域:
- 遗传学和免疫学 遗传学和免疫学
- 分子生物学分子生物学
背景情况:
- 脊髓炎 (AS) 是一种慢性炎症性疾病,主要影响脊柱.
- 在AS病变发生过程中分泌的蛋白酸性和丰富的囊蛋白 (SPARC) 的作用尚未完全理解.
- 研究SPARC的遗传关联和表达水平可能会为AS病因学提供洞察力.
研究的目的:
- 为了检查SPARC基因多态性和AS易感性在汉族中国人群之间的关联.
- 与健康对照人群相比,量化AS患者的SPARC mRNA和蛋白质表达水平.
主要方法:
- 在768名AS患者和768名对照中使用TaqMan试验在SPARC基因中对9个单核酸多态 (SNPs) 的基因定型.
- 通过实时PCR对外围血液单核细胞 (PBMC) 和粒细胞中SPARC mRNA表达的量化.
- 使用ELISA测量血清SPARC蛋白水平.
主要成果:
- 特定的SPARC等位基因 (rs171121187 A,rs4958487 G) 和单位基因 (rs17112187/rs4958487 AA,CG) 与改变的AS风险有显著联系.
- 与对照人群相比,AS患者的PBMC和粒细胞中的SPARCmRNA水平显著下降.
- 在AS患者中,SPARC蛋白水平也显著降低.
结论:
- 在SPARC基因中的多态性与对结性脊髓炎的易感性有关.
- 在来自汉族人口的AS患者中观察到SPARC mRNA和蛋白质的减少表达.
- 这些发现表明,SPARC在AS的发病过程中发挥了作用.
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