在MALDI-MSI中使用激光后电离 (MALDI-2) 提高代谢物覆盖率
J C McKinnon1, H H Milioli2,3,4, C A Purcell2,3,4
1Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Northfields Ave, Wollongong, NSW 2522, Australia. sellis@uow.edu.au.
Analytical methods : advancing methods and applications
|August 22, 2023
概括
带有激光后电离 (MALDI-2) 的矩阵辅助激光溶解/电离质谱成像显著提高了空间代谢. 这种先进的技术提高了复杂组织中低分子量代谢物的代谢物覆盖率和敏感性.
科学领域:
- 代谢学 代谢学 代谢学
- 质谱仪成像成像 质谱仪成像
- 生物化学 生物化学
背景情况:
- 矩阵辅助激光脱/电离质谱成像 (MALDI-MSI) 对于理解异质组织中的代谢变化至关重要.
- 传统的MALDI-MSI在代谢物覆盖和敏感性方面存在局限性,特别是在低分子量 (LMW) 代谢物方面.
研究的目的:
- 调查合激光后电离 (MALDI-2) 与 MALDI-MSI 在空间代谢学方面的好处.
- 评估对LMW代谢物 (<600 m/z) 代谢物覆盖率和检测灵敏度的改进.
主要方法:
- 负离子模式 MALDI-2 与 MALDI-MSI 结合对小鼠脏和肝脏组织的应用.
- 与使用特定矩阵 (例如,N-(1-naphthyl) 乙烯基二胺二) 的传统MALDI-MSI相比,MALDI-2性能的比较.
- 使用METASPACE平台对数据集进行分析,并对瘤特异性代谢物进行同定位分析.
主要成果:
- 与传统的MALDI相比,MALDI-2在小鼠脏组织中的组织特异性质量特征数量几乎翻了一番.
- MALDI-2提高了检测灵敏度,并使以前无法检测到的LMW代谢物 (例如尿素) 的成像成为可能.
- MALDI-2在脏组织中发现了34种独特的代谢物m/z特征,这些特征在公共数据集中没有得到一致的注释.
- 在转移性乳腺癌的肝脏组织中,MALDI-2检测到六种瘤特异性代谢物,而其他代谢物 (例如谷氨酸) 的信号强度增加了20倍.
结论:
- MALDI-2在空间代谢组学的灵敏度和代谢物覆盖率上提供了显著的改进.
- 这种技术为复杂的生物样本中的代谢异质性提供了前所未有的见解.
- MALDI-2代表了在研究和诊断中对代谢物成像的强大进步.
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