来自卡路里限制小鼠血清对C2C12神经管中mTOR信号传递的影响
Takanaga Shirai1,2,3, Tomohiro Iwata4, Kazuki Uemichi2,4
1Institute of Health and Sport Sciences, University of Tsukuba, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, Japan.
The Journal of endocrinology
|August 22, 2023
概括
卡路里限制 (CR) 改善新陈代谢,延长寿命,但减少肌肉质量. 然而,CR食小鼠的血液因子增强了细胞中的肌肉蛋白质合成和线粒体功能.
科学领域:
- 代谢调节 代谢调节 代谢调节
- 线粒体生物学 线粒体生物学
- 骨肌肉生理学 骨肌肉生理学
背景情况:
- 已知卡路里限制 (CR) 可以改善代谢健康并延长寿命.
- 慢性脊髓炎可能导致骨肌肉质量下降和代谢概况变化.
- 在CR期间,血液传递的因素可能会影响骨肌肉的适应.
研究的目的:
- 研究CR对骨肌肉信号传递和线粒体功能的影响.
- 为了确定CR诱导的小鼠的血清因子是否会影响骨肌肉细胞.
- 为了检查哺乳动物目标拉巴胺素 (mTOR) 信号传递在CR相关的肌肉变化中的作用.
主要方法:
- 小鼠接受了8周的随意食 (AL) 或40%的CR.
- 对CR和AL小鼠的骨肌组织进行了分析.
- 用来自AL或CR小鼠的血清治疗C2C12神经管.
主要成果:
- 在小鼠中,CR降低了肌肉质量和蛋白质合成反应.
- 与AL血清相比,用CR血清治疗的神经管显示蛋白质合成增加.
- 在接受CR血清治疗的CR小鼠和肌管中,线粒体功能得到了增强.
结论:
- 脊髓癌对骨肌肉的影响涉及直接影响和循环因素.
- 在CR期间血液中分泌的因素可以刺激肌肉蛋白质合成.
- CR促进线粒体功能,可能由血液传递的信号分子介导.
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