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在携带FLT3-ITD突变的AML患者中N-MYC癌基因表达的转移

Konstantin Bogdanov¹, Ekaterina Kudryavtseva¹, Yulia Fomicheva¹

¹Almazov National Medical Research Centre, 2 Akkuratova Str., Saint Petersburg 197341, Russia.

Pathophysiology : the official journal of the International Society for Pathophysiology

|August 22, 2023

概括

在急性髓性白血病 (AML) 中的FLT3-ITD突变会提高N-MYC瘤基因表达,独立于FLT3-ITD水平. 这表明N-MYC可能会导致AML的治疗耐药性和疾病进展.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

类似FMS的铁酶3 (FLT3) 突变在急性髓性白血病 (AML) 中很常见.
FLT3突变影响基因表达,影响白血病发生.
通常与神经母细胞瘤相关的基因在AML中研究较少.

研究的目的:

研究AML中神经母细胞瘤相关基因的表达.
评估FLT3-ITD突变与AML中的N-MYC癌基因表达之间的关系.
评估N-MYC表达与FLT3-ITD等位体负载和治疗反应相关的临床意义.

主要方法:

在初级AML患者的基因表达分析.
基于FLT3突变状态和型的N-MYC表达的比较.
在N-MYC表达和FLT3-ITD等位荷载之间的相关性分析.
在AML治疗期间对N-MYC表达的纵向监测.

主要成果:

在患有FLT3-ITD突变的AML患者中,N-MYC瘤基因表达显著更高 (超过3倍),与其他突变或正常型相比 (p = 0.03946).
与其他研究的基因不同,N-MYC表达显示出与FLT3-ITD等位基因负载的弱相关性或独立性 (p = 0.0405).
治疗后N-MYC表达的升高持续了长达三个月,尽管一些患者的FLT3-ITD等位体负载下降.

结论:

N-MYC基因上调是一种与AML中的FLT3-ITD突变相关的独特特征.
N-MYC表达似乎独立于FLT3-ITD等位体负载,这表明了替代的调节机制.
治疗后持续的N-MYC升高可能表明新出现的治疗耐药性和AML进展.

关键词:

在FLT3-ITD等位体负荷.N-MYC瘤基因表达的表达方式急性骨髓性白血病 (AML) 是一种急性骨髓性白血病.

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