相关实验视频

Updated: Aug 2, 2026

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
09:22

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

Published on: April 13, 2018

16.6K

关于血清细胞外囊泡恢复,大小分布和蛋白质组学四种净化方法的比较

Dianny Elizabeth Jimenez1, Muhammad Tahir2, Muhammad Faheem1,3

  • 1Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília 71966-700, Brazil.

Proteomes
|August 22, 2023
PubMed
概括

研究人员比较了从血清中净化细胞外囊泡 (EV) 的四种方法. 不同的净化技术产生了不同的颗粒度,大小和蛋白质含量,突出显示了对临床使用可重复的EV隔离协议的需求.

关键词:
电动汽车 (EV) 是一个电动汽车.外基因组是外基因组中的一个.细胞外囊泡细胞外囊泡质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量微微的微小的微小的微小的微小的微小分子造型分析 (MOP) 是一种分子造型分析.蛋白质组学 蛋白质组学净化方法 净化方法

更多相关视频

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media10:46

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

Published on: May 13, 2022

3.9K
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

3.1K

相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...