代谢物注释信心评分 (MACS):一种新的MSI识别评分工具
Seth M Eisenberg1, Kevan T Knizner1, David C Muddiman1
1FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States.
Journal of the American Society for Mass Spectrometry
|August 22, 2023
概括
与现有的分数相比,一种新的评分系统 - - 代谢物注释信心分数 (MACS) - - 提供了一种更可靠的方法来评估在质谱成像 (MSI) 数据中化合物识别的信心.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 分子成像学分子成像学
背景情况:
- 质谱成像 (MSI) 可视化样本中的离子分布.
- 化合物识别依赖于将质量与电荷比率 (m/z) 与数据库相匹配.
- 高分辨率,精确的质量 (HRAM) MSI数据通常使用像METASPACE这样的平台进行注释.
研究的目的:
- 引入和评估代谢物注释信心评分 (MACS) 系统.
- 为了解决HRAM-MSI数据的信任评分方面的局限性.
- 为了将MACS与METASPACE的现有代谢物-信号匹配 (MSM) 得分进行比较.
主要方法:
- 使用关键MSI指标开发了MACS评分系统:质量精度,光谱精度和空间分布.
- 描述了MACS系统的性能.
- 将MACS得分与METASPACE生成的MSM得分进行对比,用于注释的离子.
主要成果:
- 来自METASPACE的MSM得分往往缺乏一致和合理的信心水平.
- MACS为HRAM-MSI数据提供了更强大的注释信心指标.
- 该研究详细介绍了MACS的表征和比较性能.
结论:
- MACS为评估对HRAM-MSI的注释信心提供了一个优质的替代方案.
- 这种新的评分系统提高了MSI中化合物识别的可靠性.
- 在分子成像中,MACS提高了数据分析的可靠性.
关键词:
在 IR-MALDESII 中,在 MATLAB 中,我们可以使用 MATLAB.在SSIM中,SSIM就是SSIM.标注评分 标注评分 标注评分质量测量准确度 质量测量准确度频谱精度 频谱精度 频谱精度更多相关视频
17:12Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS
Published on: December 20, 2010
15.6K
09:38Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
8.6K
相关概念视频
01:19MALDI-TOF Mass Spectrometry
4.9K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
4.9K
01:33Peptide Identification Using Tandem Mass Spectrometry
6.5K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.5K