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接近红外驱动的纳米火箭用于快速和超灵敏地检测微RNA

Tong-Lin Hou¹, Xiao-Long Zhang¹, Jie Zhou¹

¹Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.

Analytical chemistry

|August 22, 2023

概括

金纳米星为近红外驱动的纳米火箭提供燃料,用于超敏感的微RNA (miRNA) 检测. 与传统的基于DNA的纳米机器相比,这种先进的纳米机器提供了更快,更有效的检测.

科学领域:

纳米技术纳米技术
生物医学工程生物医学工程
分析化学分析化学

背景情况:

传统的基于纳米材料的纳米机器 (NM) 反应时间长,效率低.
开发更快,更有效的NMS对于先进的生物传感应用至关重要.

研究的目的:

开发一种新的近红外驱动纳米火箭 (NIDNR),用于超敏感的微RNA (miRNA) 检测.
用金纳米星 (AuNSs) 和光热驱动力来增强NMS的动力和热力性能.

主要方法:

引入金纳米星 (AuNSs) 作为NIDNR的燃料核心.
使用局部表面等离子体 (LSP) 提供光热驱动力.
开发一个用于miRNA检测的电化学生物传感平台.

主要成果:

实现了对NIDNR的显著减少15分钟的反应时间,比传统的基于DNA的NMS快8倍以上.
证明了超敏感的miRNA检测,检测极限 (LOD) 低至2.95 aM.
成功地应用了NIDNR来实时测量来自人类肝细胞癌细胞 (MHCC97L) 和HeLa细胞的生物样本.

结论:

NIDNR克服了传统NMS在反应时间和效率方面的局限性.
这种创新的纳米火箭设计为敏感和快速的miRNA检测提供了一个多功能平台.
为临床样本分析和先进的生物传感构建提供了有前途的方法.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...