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在植物中,基因表达和基因编辑在莫索竹叶中

Huayu Sun¹, Sining Wang¹, Zhimin Gao²

¹Key Laboratory of State Forestry and Grassland Administration/Beijing on Bamboo & Rattan Science and Technology; Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, International Centre for Bamboo and Rattan.

Journal of visualized experiments : JoVE

|August 22, 2023

概括

一种新的竹子基因转换方法使用伤口和真空用于Agrobacterium介导的基因表达,绕过了漫长的组织培养. 这种高效的技术可以在竹子中快速进行基因编辑和功能验证.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

传统的竹子基因转换受到复杂,耗时的感应和再生的阻碍.
有效的基因表达和编辑方法对于理解竹子分子机制至关重要.

研究的目的:

为竹子开发一种新的,高效的植物基因转换方法.
建立一个快速的基因编辑系统,使用竹子中的原生记者.

主要方法:

在竹子幼苗中通过受伤和真空透,通过农细菌介导的基因表达.
使用RUBY记者和Cas9基因进行外源基因表达.
开发一种基因编辑系统,使用紫素去氧化酶 (PeVDE) 基因作为原生记者.

主要成果:

通过使用GV3101菌株实现RUBY基因表达的高转化效率 (85.2%).
成功生成了PeVDE基因的局部突变,突变率为17.33%.
通过使用PeVDE报告员,证明了 cinnamoyl-CoA减少酶基因 (8.3%的突变率) 的成功编辑.

结论:

小说中的植物方法为竹子基因转换和编辑提供了一个快速有效的替代传统组织培养方法.
编辑的PeVDE基因作为竹子外源和内源基因研究的可靠原生报告员.
这种方法加速了基因功能验证,并有助于阐明竹子中关键的代谢途径.

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