从乳糖菌 (Lactococcus lactis) 在酸乳酪中生产尼,并补充营养素
Sahana Vijayakumar1, Vishnu G1, Prajna Rao Krishnapura1
1Department of Chemical Engineering, National Institute of Technology Karnataka, Mangaluru, Karnataka, India.
Preparative biochemistry & biotechnology
|August 22, 2023
概括
研究人员利用乳球菌乳糖和酸乳糖,乳业的副产品,优化了Nisin的生产. 用酵母提取物和糖糖补充剂显著提高了尼辛的产量,证明了对食品防腐剂制造的可持续方法.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 尼辛是一种FDA批准的抗微生物,用于食品防腐剂.
- 像酸乳酪这样的工业副产品往往未得到充分利用.
- 优化发酵过程可以提高有价值的生物分子的生产.
研究的目的:
- 调查使用酸乳糖作为由乳球菌乳酸菌 (Lactococcus lactis) 生产尼辛的基质的潜力.
- 通过补充营养素和非营养因素来优化Nisin产量.
- 为了比较优化乳清介质 (OWM) 中的Nisin生产与标准的MRS介质.
主要方法:
- 乳球菌的发酵 Lactococcus lactis亚种. 乳糖ATCC® 11454使用酸乳酪作为基质.
- 优化营养补充 (酵母提取物,糖) 和中等pH值.
- 分析金属离子消耗和Nisin诱导效应.
- 在OWM与MRS介质中比较Nisin生产.
主要成果:
- 补充6%的酵母提取物和4%的糖糖显著提高了尼辛的生产.
- 优化乳清介质 (OWM) 与MRS介质相比支持更高的Nisin产量.
- 添加尼辛 (0.22毫克/毫升) 作为诱导剂,进一步促进了自身的产生.
- 铜离子被确定为发酵过程中消耗最多的金属离子.
- 在OWM中,获得的最高Nisin产量是2.6 × 10^5AU/mL.
结论:
- 酸乳酪是尼辛生产的可行且具有成本效益的基质.
- 营养素优化和理解发酵参数对于最大限度地提高尼辛产量至关重要.
- 这项研究提出了一种可持续的方法,用工业废物生产一种有价值的食品防腐剂.
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