Bcl6,Irf2和Notch2促进非经典单细胞的发展
Kevin W O'Connor1, Tiantian Liu1, Sunkyung Kim1
1Department of Pathology and Immunology, Washington University in St. Louis, School of Medicine, St. Louis, MO 63110.
NOTCH2信号传递,由三角形状联体1 (DLL1) 触发,促进Ly6Clo单细胞从Ly6Chi单细胞的发展. 这个过程需要IRF2,并影响TREML4的表达,揭示了一个转录层次结构.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- Ly6Clo单细胞对于血管内皮的监测至关重要.
- 已知Ly6Clo单细胞是从Ly6Chi单细胞中发展出来的.
- NOTCH2信号传递与Ly6Clo单细胞发育有关,但潜在的机制尚不清楚.
研究的目的:
- 为了研究NOTCH2信号传导在Ly6Clo单细胞发育中的髓状细胞原始体中的作用.
- 阐明Ly6Clo单细胞分化的转录要求.
主要方法:
- 在体外培养骨髓原始体的培养.
- NOTCH2信号诱导使用类似delta的连接体1 (DLL1).
- 对单细胞子集过渡和基因表达的分析 (TREML4,BCL6,IRF2,NUR77).
主要成果:
- DLL1诱导的NOTCH2信号促进了Ly6Chi TREML4-单细胞向Ly6Clo TREML4+单细胞的过渡.
- 删除BCL6取消了Ly6C单细胞的发展.
- IRF2对于Ly6Clo单细胞以细胞内在的方式发展至关重要.
- DLL1诱导的转换需要IRF2,但不一定是BCL6或NUR77.
结论:
- 通过DLL1发送NOTCH2信号,驱动Ly6C单细胞的发展.
- IRF2是Ly6Clo单细胞分化的关键转录因子.
- 一个涉及BCL6,IRF2和NUR77的转录层次结构调节了Ly6C单细胞的发育.
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