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在正规的莉花信号传递中,一个持久的主要突变嵌入了由草食引起的基因网络

Rishav Ray¹, Rayko Halitschke¹, Klaus Gase²

¹Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.

Proceedings of the National Academy of Sciences of the United States of America

|August 22, 2023

概括

植物对昆虫食草动物的防御依赖于斯酸盐 (JA) 信号传递. 在Nicotiana attenuata中JA-Ile生物合成基因NaJAR4的突变影响了植物的健康和防御,由于网络补偿而持续存在.

科学领域:

植物生物学植物生物学
生态生态学生态生态学
遗传学是一个遗传学.

背景情况:

昆虫的草食会触发植物的防御反应,这种反应由斯酸盐 (JA) 信号传导,特别是斯-L-异氨酸 (JA-Ile) 爆发.
尼科蒂亚纳度 (Nicotiana attenuata) 作为研究这些植物-草食动物相互作用和防御机制的模型系统.

研究的目的:

研究JA-Ile生物合成中的自然变异及其与植物健康和防御的联系.
了解植物对昆虫口腔分泌物 (OS) 的反应的基因架构和监管网络.

主要方法:

利用Nicotiana attenuata的MAGIC种群和更新的基因组组件来识别OS引起的JA-Ile爆发中的自然变异.
分析了长达十年的种子收集,以评估基因变异的频率和进化维护.
进行了转录组和代谢组的整合性分析,以及在共同表达网络中的基因沉默实验.

主要成果:

鉴定了NaJAR4基因的突变,在没有草食动物的情况下与更高的健康状况相关,但损害了叶子防御.
发现两个NaJAR同类 (4和6) 具有互补的空间和时间功能.
发现了通过平衡选择维持的酶性不活跃变异,并揭示了NaJAR4是复杂基因联合表达网络的一部分.

结论:

具有显著适应性影响的突变可以通过补偿基因网络反应在自然种群中持续存在.
这种遗传补偿促进了保留信号通路内的多样化,与全基因模型保持一致.
这项研究强调了遗传变异,基因网络和植物适应草食之间的复杂相互作用.

关键词:

魔法人群中的魔法人群在 NaJAR4 中.莉花的信号传递.自然突变具有很大的健康影响.工厂防御系统的工厂防御系统

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