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希斯脱酶6与无酸不敏感5的相互作用降低了果对干旱的耐受性

Wenjie Li¹, Mengting Deng¹, Shicong Wang¹

¹State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.

Plant physiology

|August 22, 2023

概括

果组织基因脱乙酶6 (MdHDA6) 对干旱耐受性产生负面调节. 它抑制干旱反应基因,包括由酸信号因子MdABI5调节的基因,影响植物的水应激弹性.

科学领域:

植物分子生物学植物分子生物学
植物生理学植物生理学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

植物对干旱的耐受性对农业至关重要,需要分子理解才能进行改进.
基因失乙酶在基因调节中发挥作用,但它们在果干旱反应中的具体功能尚不清楚.

研究的目的:

描述果基素脱乙酶6 (MdHDA6) 在耐旱性中的作用.
阐明MdHDA6调节干旱反应基因的分子机制.

主要方法:

产生具有改变MdHDA6表达的转基因果植物.
转录和ChIP-seq分析以评估基因表达和基因素乙化.
通过共免疫沉和双露西法酶试验研究蛋白质与蛋白质相互作用.

主要成果:

过度表达MdHDA6降低了干旱耐受性,而其下调则增强了它.
MdHDA6促进干旱反应性基因上的基因脱乙烯化,导致它们的抑制.
MdHDA6与MdABI5相互作用,形成一个抑制ABA响应基因的复合体,包括MdRD29A.

结论:

在果中,MdHDA6通过抑制干旱反应基因,通过基因素脱乙烯化来负面调节干旱耐受性.
MdHDA6-MdABI5复合体为果的干旱压力反应提供了一个新的调节途径.
这项研究提供了一个分子基础,通过针对性基因操纵来增强果的抗干旱能力.

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