查询8: 2甲基烯酸的体内蛋白质组氨酸活性
David Ross Hall1,2, Jeremy Gauthier1, Hui Peng1,2
1Department of Chemistry, University of Toronto, Toronto, Ontario M5S 1A1, Canada.
Environmental science & technology
|August 22, 2023
概括
在织涂料中使用的甲烯酸 (FTACs) 可以与细胞醇反应,导致蛋白质聚合和潜在的毒性. 这项研究确定了100多种改性,表明FTACs对健康构成风险.
科学领域:
- 环境化学环境化学
- 毒理学 毒理学 毒理学
- 生物化学 生物化学
背景情况:
- 传统的甲基和多甲基物质 (PFAS) 正在逐步淘汰,但基于甲基聚合物 (FTP) 的聚合物仍在使用,特别是用于织涂料.
- FTPs可以降解为遗留的PFAS并释放甲烯酸 (FTAC) 单体,引发健康问题.
- FTAC单体可能会与细胞醇发生反应,这种毒性机制需要研究.
研究的目的:
- 为了研究甲烯酸 (FTAC) 单体与细胞醇的潜在反应性.
- 为了在体外识别和描述由FTACs形成的蛋白质添加物.
- 探索FTAC修改对蛋白质功能的影响,特别是蛋白质聚合.
主要方法:
- 利用固相提取 (FSPE) 来丰富FTAC修饰的.
- 采用修改的纳米液体染色学与自下而上的数据依赖蛋白质学相结合,用于识别.
- 使用合成C6F13-乙胺 (F13-IAM) 化学探针来评估最大的PFAS-thiol反应性.
主要成果:
- 鉴定出超过100种由8:2托烯酸 (8:2 FTAC) 共同修饰的独特.
- 鉴定到的 8:2 FTAC 修饰的氨酸残留物中,没有一个被注释为活性部位核友.
- 观察到 8:2 FTAC 修饰在体外促进了蛋白质聚合,使用 F13-IAM 检测到超过 700 种修饰的.
结论:
- 8:2 FTAC与蛋白质二醇具有显著的反应性.
- FTAC的修改可能导致蛋白质聚合,这表明PFAS诱导的毒性潜在的一般机制.
- 需要进一步的研究,以了解FTAC暴露的全部毒理影响.
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