阿拉比多普西斯的口腔系细胞建立双极性并分离差异信号传导能力,以调节干细胞的潜在信号
Eva-Sophie Wallner1, Liam Dolan2, Dominique C Bergmann3
1Department of Biology, Stanford University, Stanford, CA 94305-5020, USA; Gregor Mendel Institute, Dr.-Bohr-Gasse 3, 1030 Wien, Austria; Howard Hughes Medical Institute, Stanford, CA 94305, USA.
Developmental cell
|August 22, 2023
概括
研究人员在植物中发现了一个新的极性领域,它控制细胞分裂和命运. 这一域涉及八像蛋白质,有助于在阿拉比多普西斯口腔系中产生细胞多样性.
科学领域:
- 植物生物学 植物生物学
- 细胞生物学 细胞生物学
- 发育生物学是发展生物学.
背景情况:
- 细胞极性和不对称的细胞分裂 (ACD) 对于产生细胞多样性至关重要.
- 在Arabidopsis中,BASL蛋白标记着一个极性域,该极性域指导口腔系中的ACDs,指导细胞命运.
- 控制细胞命运决定和植物发育中的分裂模式的机制是复杂的,并积极研究.
研究的目的:
- 识别和表征参与调节细胞分裂和细胞命运的新型极性域在阿拉比多普西斯口腔系.
- 在不对称的细胞分裂中研究像蛋白 (OPL) 的功能和调节.
- 探索OPL蛋白在植物发育中的进化保护和潜在的古代功能.
主要方法:
- 在Arabidopsis中进行基因分析,以研究OPL蛋白质的作用.
- 错误表达研究,观察改变的OPL水平对细胞分裂和命运的影响.
- 同焦显微镜用于跟踪在不对称细胞分裂期间的OPL蛋白定位.
- 涉及肝Marchantia的异位表达的比较研究,以评估进化保护.
主要成果:
- 发现了第二个,相反位置的极性域,标记为Octopus-LIKE (OPL) 蛋白质,先于ACD.
- OPL 蛋白质分离到较小的美里斯形子细胞中.
- OPLs促进了美里斯蒂-放大分裂,并延迟了向口腔命运的进展.
- OPL的功能和局部化在很大程度上独立于其他已知的口腔极性基因和布拉西诺固醇信号传递.
- 在Marchantia中OPLs的子宫外表达抑制了表皮命运的进展,表明保留了调节作用.
结论:
- OPL蛋白质代表了一个新的极性域,在调节细胞分裂和Arabidopsis口腔系的命运方面发挥着关键作用.
- 在此背景下,OPL介导的途径与先前识别的极性机制和类固醇信号传递有所区别.
- OPL参与了植物进化过程中保存的古老途径,以控制细胞分裂和命运,这表明它在植物发育中发挥了基本作用.
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