血清成分影响抗体对甘氨酸和DNA抗原的反应性
1Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, 305-8566, Japan. t-okuda@aist.go.jp.
Scientific reports
|August 22, 2023
概括
哺乳动物血清成分显著改变抗体对碳水化合物抗原的反应性,包括DNA. 净化过程可以改变抗体的特异性,突出显示血清.
科学领域:
- 免疫学 免疫学 免疫学
- 葡萄糖生物学 葡萄糖生物学
- 生物化学 生物化学
背景情况:
- 此前已经产生了针对特定甘氨酸的单克隆IgM抗体.
- 在抗甘氨酸IgM抗体和已知的抗DNA抗体之间观察到序列同质性.
研究的目的:
- 研究抗甘氨酸IgM抗体与DNA的交叉反应性.
- 确定血清成分对抗体活性和特异性的影响.
主要方法:
- 酶相关免疫吸收试验 (ELISA) 用于评估抗体对DNA的反应性.
- 柱色谱用于抗体净化.
- 从小鼠模型 (NZB/W F1,MRL/lpr) 和人类血清中分析了纯化的抗体.
主要成果:
- 抗α2,6-sialyl LacNAc IgM显示与DNA的交叉反应,在净化后增加.
- 净化消除了污染的牛血清蛋白质,表明它们对抗体活性的影响.
- 来自各种来源的纯化抗DNA抗体表现出改变的抗原结合特异性.
结论:
- 哺乳动物血清含有成分,可显著调节对碳水化合物抗原的抗体活性,包括DNA.
- 抗体特异性可以在从含血清制剂的净化过程中改变.
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