癌基因Musashi1编码了乳腺癌中的新型miRNA
Liana Lachinani1, Mahboobeh Forouzanfar1, Kianoush Dormiani2
1Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Scientific reports
|August 22, 2023
概括
研究人员在Musashi1 (MSI1) 基因中发现了两个新的微RNA (MSM2和MSM3). 这些microRNAs在乳腺癌细胞中表达,可能在癌症发展中发挥作用.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 在瘤学瘤学.
背景情况:
- 穆沙希1 (MSI1) 是一种RNA结合蛋白,在各种癌症中表达高,表明其作为预后标记物的潜力.
- 假设MSI1基因可能编码具有癌症发展或生物标志物作用的微RNA (miRNA) 需要进行调查.
研究的目的:
- 为了识别MSI1基因内部区域内编码的新型miRNAs.
- 描述这些新型MSI1编码miRNAs在癌症中的表达和潜在功能.
主要方法:
- 在MSI1内部的miRNA前体的生物信息预测.
- 将前体miRNAs转移到HEK293T细胞中,以确认成熟miRNAs的外源表达.
- 在癌细胞系 (MCF-7,SH-SY5Y) 和临床乳腺癌样本中检测内源性miRNA表达.
- 双 luciferase 试验用于验证新型 miRNA 与其目标基因 (PDE11A) 之间的相互作用.
主要成果:
- 两个新的miRNA前体MSM2和MSM3在MSI1基因的内突4内被确定.
- MSM3前体产生了两个成熟的miRNA (MSM3-3p和MSM3-5p),而MSM2前体产生了一个 (MSM2-5p).
- 在乳腺和神经母细胞瘤细胞系以及临床乳腺癌样本中证实了MSM2-5p和MSM3-3p的内源表达.
- 已经证明MSM3-3p与PDE11A基因的3'UTR相互作用.
结论:
- 该MSI1基因编码至少两个新型miRNAs,MSM2和MSM3.
- 这些新型miRNAs在乳腺癌细胞中表达,这表明它们可能在恶性瘤中发挥作用.
- 这些发现为了解MSI1在癌症中的作用和探索新的miRNA生物标志物开辟了新的途径.
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