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丸mRNA表达水平的调节失调在化场生产的与野生大珀 Seriola dumerilili之间的比较
Anna Lavecchia1, Caterina Manzari1, Chrysovalentinos Pousis2
1Department of Biosciences, Biotechnologies and Environment, University of Bari Aldo Moro, Via Orabona 4, 70124, Bari, Italy.
Scientific reports
|August 22, 2023
概括
在囚禁中养殖的雄性大珀 (Seriola dumerili) 呈现出生殖功能障碍. 转录组分析揭示了丸中广泛的基因表达变化,表明即使在"正常"养殖鱼中,生物过程受到干扰和精子生成受损.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 鱼类生殖生物学 鱼类生殖生物学
- 分子生物学分子生物学
背景情况:
- 繁殖功能障碍越来越多地观察到来自野生种群和囚禁养殖的雄性大珀 (Seriola dumerili).
- 囚禁育种计划旨在减轻生殖问题,但它们在更大的珀鱼中的成功仍在调查中.
研究的目的:
- 为了比较野生雄性大珀鱼的丸转录组与表现出正常和功能障碍的精子生成的化场生产的鱼.
- 确定受养鱼生殖功能障碍影响的分子通路和生物过程.
主要方法:
- 对来自三个群体的丸进行了转录组分析 (基因表达概况):野生 (WILD),明显正常的养殖 (NormalF) 和功能障碍的养殖 (DysF).
- 进行了差异基因表达分析,以确定群体之间的显著基因表达变化.
- 用基因丰富分析来确定受影响的生物途径.
主要成果:
- 数以千计的差异表达基因 (DEGs) 在比较中被确定,在功能失调的养殖鱼 (DysF) 中存在显著的失调.
- 在DysF鱼类中,破坏的生物过程包括细胞组装,类固醇生成和细胞亡,在卵细胞成熟和细胞循环途径中进行丰富.
- 即使是"正常"的养殖鱼 (NormalF) 也显示了关键基因的下调,包括参与细胞亡调节的ptpn6,这表明了潜在的功能障碍.
结论:
- 养大珀,即使是从养养的蛋中养,也不能防止生殖功能障碍.
- 养殖场雄性大珀的繁殖问题涉及多个生物过程和代谢途径的复杂干扰.
- 该研究强调,需要进一步研究养殖鱼类生殖功能障碍背后的分子机制.
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