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Updated: Jul 18, 2025

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实时PCR方法用于评估小型循环生产中ParA介导的重组效率

Cláudia P A Alves1,2, Duarte Miguel F Prazeres1,2, Gabriel A Monteiro3,4

  • 1iBB- Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.

Methods in molecular biology (Clifton, N.J.)
|August 22, 2023
PubMed
概括

一个新的实时PCR协议准确地测量了父母等离子体重组的效率. 这种方法比传统的凝分析提供了更可重复的结果,用于创建小圆圈.

关键词:
内部分子重组的分子内组合.迷你圈子 迷你圈子在 ParA 解决方案中,实时PCR可以实现.重组效率的效率重新组合.

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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