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针对位点定向突变的逆PCR

Diogo Silva1, Gustavo Santos2, Mário Barroca2

  • 1Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade NOVA de Lisboa, Oeiras, Portugal.

Methods in molecular biology (Clifton, N.J.)
|August 22, 2023
PubMed
概括

反向PCR可以使用定制的原料引入精确的DNA突变. 这种方法通过放大和修改循环DNA来促进基因工程,这对于生物和生物技术研究至关重要.

关键词:
反向PCR是一种反向PCR.没有重叠的原料.蛋白质工程是一种蛋白质工程.局部导向的突变发生.

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