相关实验视频

Updated: Jul 18, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

生物工程化学tRNA/pre-miRNA作为癌症治疗中的前药物

Fatemeh Rafieenia¹, Seyed Omar Ebrahimi², Ensieh Sadat Emadi²

¹Department of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

Biotechnology progress

|August 23, 2023

概括

生物工程微RNA (miRNA) 提供了比合成版本更安全,更有效的癌症治疗方法. 微生物发酵可以经济地生产这些生物产药,用于向癌症治疗.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物技术是生物技术.

背景情况:

非编码RNAs (ncRNAs),特别是microRNAs (miRNAs),在癌症病理学和瘤发生中起着至关重要的作用.
目前基于miRNA的癌症疗法主要依赖于化学合成的miRNA,其安全性和生物活性可能受到限制.
生物工程miRNAs为合成模仿物提供了替代品,可能克服与折叠,安全性和有效性相关的问题.

研究的目的:

审查用于癌症治疗的生物工程微RNA (miRNA) 的进展.
探索生物工程miRNAs作为针对癌症治疗的生物预药的潜力.
讨论生物工程miRNA疗法领域的新方法,包括仿真tRNA/预miRNA.

主要方法:

使用微生物发酵来经济地生产生物工程miRNAs.
在癌细胞中进行选择性处理,开发生物工程化化学miRNAs.
研究各种载体的使用,以有效地传递生物工程miRNAs.

主要成果:

通过微生物发酵生产的生物工程miRNAs提供了一个具有成本效益和潜在的更安全的替代合成miRNAs.
化学miRNAs可以被设计为针对性传递和选择性激活在癌细胞内.
这种方法对开发新型,高度特异性的癌症疗法充满希望.

结论:

生物工程miRNAs代表了癌症治疗的有希望的新途径,提供了更好的安全性和特异性.
通过微生物发酵经济生产使这种方法可扩展和可访问.
为了推进癌症治疗策略,对生物工程化基因组miRNAs,包括tRNA/pre-miRNA结构的进一步研究是有必要的.

关键词:

生物工程 ncRNAs 的生物工程.癌症治疗疗法癌症治疗仿真tRNA/预miRNAsss可以使用.作为一种前药物,它是一种前药物.

更多相关视频

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

Published on: February 21, 2025

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Published on: August 23, 2024

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Microorganisms in Medicine and Therapeutics

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.