在患有冠状动脉样硬化症的患者中,对外周血液单细胞的差异表达基因的探索性研究
Juhai Chen1,2, Fengyan Xu3, Xiangang Mo3
1Guizhou Medical University, Guiyang, 550004, Guizhou Province, People's Republic of China.
Combinatorial chemistry & high throughput screening
|August 23, 2023
概括
研究人员确定了五个关键基因作为诊断动脉样硬化 (CAS) 的潜在生物标志物. 这一发现可能会导致针对这种常见疾病的新向治疗方法.
科学领域:
- 心血管研究研究心血管研究
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 循环单细胞的丰富性与人类的动脉样硬化发展有关.
- 动脉样硬化 (CAS) 构成严重的健康风险,需要改进诊断和治疗策略.
研究的目的:
- 为了识别动脉动脉样硬化 (CAS) 的诊断生物标志物.
- 探索CAS.潜在的向治疗策略.
主要方法:
- 在单细胞数据上使用权重基因共同表达网络分析 (WGCNA) 进行转录学分析.
- 差异基因表达分析和机器学习算法 (LASSO,SVM-RFE) 用于生物标记物识别.
- 使用公共数据库构建监管网络和药物目标预测.
主要成果:
- 五个差异表达的单细胞相关枢纽基因被确定为CAS的显著预测因子.
- 在CAS患者中,HMGB1被上调,而CCL3,CCL3L1,CCL4和DUSP1被下调.
- 涉及转录因子,miRNA和lncRNA的调控网络围绕关键诊断分子构建.
- 确定了11种潜在的药物,包括ibrutinib,以向这些生物标志物.
结论:
- 一个由五个基因生物标志物组成的小组已被确定用于CAS诊断.
- 这些生物标志物为CAS治疗中的新型治疗干预提供了潜在的目标.
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