使用5'-脱氧核化物作为生长基质通过肠外病原体E. 大肠杆菌通过二氧酸短路
Katherine A Huening1, Joshua T Groves1, John A Wildenthal1
1The Ohio State University Department of Microbiology, Columbus, OH, 43210.
bioRxiv : the preprint server for biology
|August 23, 2023
概括
致病性大肠杆菌中的DHAP分离器主要吸收用于生长的S-adenosyl-l-methionine (SAM) 的副产品,而不是回收 metionin或排毒化合物. 这一途径支持在肠外环境中的有氧生长.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 有机体使用S-adenosyl-l-methionine (SAM) 进行重要的细胞过程,产生5'-甲基腺 (MTA) 和5'-脱氧腺 (5dAdo) 等副产品.
- 二氧酸 (DHAP) 旁路是已知用于代谢MTA和5dAdo的细菌途径,以前认为它有助于甲氨酸的合成或排毒.
研究的目的:
- 调查DHAP分流在肠外致病性大肠杆菌 (ExPEC) 中的生理作用.
- 为了确定DHAP分流是否在甲氨酸合成,解毒或ExPEC中作为生长基质通路发挥作用.
主要方法:
- 用MTA和5dAdo作为基质在ExPEC中分析DHAP分流活动.
- 代谢分析,以追踪硫成分的命运,并确定增长支持.
主要成果:
- ExPEC DHAP分流器主要用于MTA和5dAdo的碳同化,以促进生长.
- 来自MTA的硫没有被显著地回收成甲因,而是以2-甲基乙醇的形式积累.
- 该途径支持有氧生长,表明在血液和尿液等有氧环境中发挥作用.
结论:
- 在ExPEC中的DHAP突变对于能量代谢和SAM副产品利用的碳同化至关重要.
- 这项研究揭示了DHAP突变在细菌病原和新陈代谢中的被忽视的功能.
- 在其他细菌物种中也可能存在DHAP分流的类似作用.
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