相关实验视频

Updated: Jul 18, 2025

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

10.9K

对Gαq-PLCβ相互作用的时空光学控制

Sithurandi Ubeysinghe, Dinesh Kankanamge, Waruna Thotamune

    bioRxiv : the preprint server for biology
    |August 23, 2023
    PubMed
    概括

    研究人员开发了Opto-dHTH,这是一种控制GαqGTP-PLCβ相互作用的光遗传工具. 这一创新揭示了RhoGEFs和PLCβ之间的分子竞争决定了Gq-GPCR驱动的细胞迁移.

    更多相关视频

    Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation08:00

    Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

    Published on: October 4, 2024

    620
    Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist07:48

    Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

    Published on: April 25, 2018

    6.3K

    相关概念视频

    Activation and Inactivation of G Proteins01:22

    Activation and Inactivation of G Proteins

    Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
    7.3K
    G-Protein Gated Ion Channels01:21

    G-Protein Gated Ion Channels

    GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
    Sensory...
    4.6K
    IP3/DAG Signaling Pathway01:11

    IP3/DAG Signaling Pathway

    Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
    12.2K
    GPCRs Regulate Adenylyl Cylase Activity01:09

    GPCRs Regulate Adenylyl Cylase Activity

    Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
    5.7K
    G-protein Coupled Receptors01:21

    G-protein Coupled Receptors

    G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
    120.2K
    Transducer Mechanism: G Protein–Coupled Receptors01:30

    Transducer Mechanism: G Protein–Coupled Receptors

    G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
    GPCRs are also called heptahelical,...
    2.1K