菌根细菌形成的细胞内脂质包容是一种与快速复制相关的动态过程
bioRxiv : the preprint server for biology
|August 23, 2023
概括
在活跃复制过程中,Mycobacterium结核菌形成了细胞内脂质包容 (ILI),独立于压力. 这表明LI的形成不仅仅与非复制性持久性有关.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 病变的发生和发病.
背景情况:
- 细胞内脂质包容 (ILI) 是菌根菌中的富含三糖的细胞器,主要与能量储备有关.
- 传统上,LI的形成与在宿主模仿压力条件下由dosR介导的过渡到非复制性持久性 (NRP) 相关.
- 在感染期间观察到富含ILI的结核菌菌 (Mtb),突出显示了它们在发病过程中的作用.
研究的目的:
- 研究菌根杆菌中ILI形成的动态和调节,特别是在活跃复制过程中.
- 确定LI的形成是否完全依赖于环境压力因素或NRP.
- 探索临床Mtb分离物之间的ILI产生的变化及其与dosR表达的相关性.
主要方法:
- 在菌培养物中培养Mycobacterium marinum和临床Mtb分离物.
- 在新鲜介质和外源性脂肪酸的反应中跟踪ILI形成动态.
- 对60个临床Mtb分离物进行ILI生产变异的查.
- 评估与ILI积累相关的dosR表达水平.
主要成果:
- 在活跃复制过程中,Mycobacterium marinum和临床Mtb分离物形成ILI,独立于环境压力因素.
- 在引入新鲜介质或脂肪酸时,ILI的形成很快,但在活跃复制过程中很快就会耗尽.
- 细胞外在静止阶段作为中性脂质积累的替代地点.
- 在Mtb的临床分离物和谱系之间存在着ILI产生的显著差异.
- dosR表达水平与临床隔离物中的ILI积累不一致.
结论:
- ILI形成是复制菌根菌的活跃过程,不仅仅局限于非复制性持久性.
- 这表明ILI形成与dosR介导的NRP途径脱.
- 环境线索和细菌生理学影响ILI动态和积累地点.
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