CASP15冷EM蛋白质和RNA点:使用实验地图进行细化和分析

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Updated: Jul 18, 2025

Cryo-EM and Single-Particle Analysis with Scipion
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Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

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Thomas Mulvaney, Rachael C Kretsch, Luc Elliott

    bioRxiv : the preprint server for biology
    |August 23, 2023
    PubMed
    概括

    用冷电子显微镜 (cryo-EM) 密度图来评估CASP15中的蛋白质模型,显示高精度,但侧链和循环定位存在挑战. 化EM图的精细化改善了蛋白质和RNA目标的模型质量.

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    Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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