用商业仪器生成的微生物单细胞地图
bioRxiv : the preprint server for biology
|August 23, 2023
概括
EASi-seq可实现高质量的单个微生物基因组测序,创建详细的微生物群地图. 这种可访问的方法有助于发现微生物亚群,并改进基因组分析.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 单细胞测序对于分析复杂的生物系统至关重要,但对微生物缺乏可访问的工具.
- 商业仪器广泛用于哺乳动物单细胞分析,突出显示微生物应用的差距.
研究的目的:
- 引入EASi-seq (易于访问的单个微生物测序),这是一种用于高效的单个微生物基因组测序的新方法.
- 通过单细胞分析,为人类和环境微生物组生成详细的基因组图谱.
主要方法:
- 针对微生物应用,调整了商业 Mission Bio Tapestri 仪器的单细胞工作流程.
- 开发了一种用于聚类微生物的伴侣生物信息管道,改进了基因组组装,菌株识别,分类学分类和基因注释.
- 集成的元基因组与EASi-seq数据相结合,以减轻捕获偏差并增强基因组覆盖范围.
主要成果:
- 通过EASi-seq,每次运行可以对数千种微生物进行测序,从而产生大型的射击枪基因组数据集.
- 展示了人类和环境微生物组的详细地图集的生成.
- 通过生物信息管道和数据集成,展示了改进的全基因组组,菌株识别,分类学分类和基因注释.
结论:
- EASi-seq为微生物组研究提供了高质量的,可访问的单细胞基因组数据解决方案.
- 该方法为发现和分析微生物物种亚群开辟了新的途径.
- 在商用平台上为单个微生物提供全面的基因组分析.
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