永生化介质干细胞 过度表达α-1抗素 保护阿基纳细胞免受阿波和费罗细胞死亡
Sara Shoeibi1, Erica Green1, Hua Wei1
1MUSC.
Research square
|August 23, 2023
概括
不朽化的干细胞通过抑制亡和铁亡来防止胰腺炎诱导的细胞死亡. 这些发现支持用于慢性胰腺炎治疗的干细胞疗法.
科学领域:
- 胃肠病学 胃肠病学
- 细胞生物学 细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 慢性胰腺炎 (CP) 涉及炎症和功能障碍.
- 初级介质干细胞/干细胞 (MSCs) 具有治疗潜力,但经历衰老.
- 永生化MSCs (iMSCs) 为克服衰老限制提供了一个潜在的解决方案.
研究的目的:
- 为了产生和表征不朽的人类MSCs (iMSCs) 和α-1抗素过度表达MSCs (iAAT-MSCs).
- 评估iMSCs和iAAT-MSCs在体外对2,4,6-Trinitrobenzenesulfonic acid (TNBS) 诱导的细胞死亡的保护作用.
- 研究涉及亡和铁亡的潜在机制.
主要方法:
- 使用猿类病毒40大T抗原 (SV40LT) 进行初级MSCs的不朽化.
- 对iMSC和iAAT-MSC线路的表征,以扩散,衰老,表型和差异化.
- 评估TNBS诱导的亡,铁亡,ER压力,线粒体功能和结完整性在培系统中.
主要成果:
- iMSCs和iAAT-MSCs抑制了TNBS诱导的早期亡,并减少了ER压力.
- 这些细胞保留了紧密结合蛋白,调节了线粒体呼吸,ATP含量和ROS产生.
- 通过调节FTH1/PDI/GPX4通路和调节ROS和铁代谢,iMSCs和iAAT-MSCs改善了铁亡.
结论:
- 铁亡被确定为TNBS诱导的细胞死亡的关键机制.
- 不朽化MSCs显示显著的保护作用,防止CP相关的细胞损伤.
- 这些发现为慢性胰腺炎的干细胞治疗提供了机械洞察力.
相关概念视频
01:19Mesenchymal Stem Cells
4.8K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.8K
01:23Tissue Renewal without Stem Cells
1.7K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
1.7K