肺状细胞癌中的烟草烟雾对瘤抑制剂PTEN的调节,基于生物信息学分析
Vladimir O Pustylnyak1,2, Efim Y Alekseenok2, Alina M Perevalova1
1Novosibirsk State University, 630090, Novosibirsk, Russia.
Heliyon
|August 23, 2023
概括
烟草烟雾降低了肺状细胞癌 (LUSC) 中的瘤抑制剂PTEN的调节. 吸烟导致的hsa-mir-301a表达的增加似乎是导致LUSC中PTEN下调的机制.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 在染色体10 (PTEN) 上被删除的酸酶和张素同源是一种关键的瘤抑制剂,在人类癌症中经常被禁用.
- 在肺平细胞癌 (LUSC) 中观察到PTEN变化,但烟草烟雾对LUSC中PTEN调节的影响仍然不太清楚.
研究的目的:
- 调查烟草烟雾对LUSC中的PTEN表达的影响.
- 探索hsa-mir-301a在调解吸烟相关的LUSC中的PTEN下调中的作用.
主要方法:
- 使用公共数据库对LUSC患者PTEN表达和吸烟状况的生物信息分析.
- 使用RT-PCR分析对LUSC患者样本进行生物信息学发现的验证.
- 在微RNA参与PTEN调节的in silico预测.
主要成果:
- 与正常组织相比,LUSC组织中观察到PTEN显著下调,与吸烟暴露相关.
- 在分析中,hsa-mir-301a被认为是吸烟LUSC患者中PTEN的潜在调节剂.
- 通过RT-PCR,在吸烟者的LUSC组织中证实了降低PTEN和增加hsa-mir-301a的表达,PTEN和hsa-mir-301a水平之间存在负相关性.
结论:
- 烟草烟雾引起的hsa-mir-301a的上调有助于LUSC中PTEN的下调.
- 这种涉及PTEN和hsa-mir-301a的机制可能在LUSC瘤发生中发挥重要作用.
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