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使用生物信息学分析识别与心力衰竭中氧化压力相关的枢纽基因及其与免疫透的相关性

Jianjun Gu^1,2, Li Na Zhang³, Xiang Gu²

¹Department of Cardiology, Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu, China.

|August 23, 2023

概括

这项研究确定了五个关键基因 (Mapk14,Hif1a,Myc,Hsp90ab1,Hsp90aa1) 与氧化应激和心力衰竭 (HF) 中的免疫反应有关. 这些基因为HF治疗和免疫治疗提供了潜在的新治疗点.

科学领域:

心血管生物学心血管生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

心力衰竭 (HF) 与氧化应激和免疫系统调节障碍显著相关.
确定关键的分子参与者,将这两种病理过程联系起来,对于开发有效的治疗方法至关重要.

研究的目的:

通过生物信息学和实验验证,识别与氧化应激和心力衰竭中的免疫透相关的枢纽基因.
探索已识别的枢纽基因与免疫细胞透到心脏组织之间的相关性.

主要方法:

对GSE36074数据集的生物信息分析,以确定与氧化应激相关的差异表达基因 (DEGs).
使用STRING和Cytoscape构建蛋白质-蛋白质相互作用 (PPI) 网络并对枢纽基因进行查.
使用CIBERSORTx对免疫细胞透的分析以及与枢纽基因的相关性分析.
使用RT-qPCR,免疫组织化学 (IHC) 和西布洛特的中心基因的实验验证.

主要成果:

确定了136种与氧化压力相关的DEG (OSRDEG),这些DEG富含了HIF-1,FoxO,MAPK和TNF信号传递等途径.
五个枢纽基因 (Mapk14,Hif1a,Myc,Hsp90ab1,Hsp90aa1) 被精确地确定.
在枢纽基因和特定免疫细胞 (例如Th2细胞,单细胞,NK细胞) 之间发现了显著的相关性.
实验验证证了这五个枢纽基因在心力衰竭中的作用.

结论:

Mapk14,Hif1a,Myc,Hsp90ab1和Hsp90aa1被确定为心力衰竭中的关键枢纽基因,在氧化应激中发挥作用.
这些基因代表了心力衰竭的潜在治疗点.
进一步研究针对这些基因的免疫疗法是有必要的.

关键词:

网络类型xx 心脏衰竭是因为心脏衰竭.枢纽基因枢纽基因枢纽基因免疫系统的透.氧化应激是一种氧化应激.

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