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在使用超高性能超临界流体色谱-质谱学时控制聚乙烯甘醇的正离子电喷气电离

Sergio Cancho-Gonzalez¹, Paul Ferguson², Julie M Herniman¹

¹Faculty of Engineering & Physical Sciences, School of Chemistry, University of Southampton, Southampton, UK.

Journal of separation science

|August 23, 2023

概括

这项研究通过使用超临界流体染色学和质谱学来简化药品中的聚乙烯糖醇 (PEG) 的分析. 该方法选择性地电离PEG,使数据分析和批量比较更容易.

科学领域:

聚合物化学聚合物化学
分析化学分析化学
制药科学制药科学

背景情况:

聚乙烯糖醇 (PEG) 是药物配方中的关键辅助成分,增强药物的溶解性和输送.
在质谱学中,PEG寡合体的非选择性电离使分析复杂化,特别是随着链条长度的增加和多个带电物种.
目前的分析方法与PEG电离的复杂性作斗争,阻碍了准确的表征和批量对批量的一致性.

研究的目的:

开发一种选择性电离方法,用于聚乙烯糖醇 (PEGs) 使用超临界流体染色体质谱法 (SFC-MS).
简化PEG质谱,促进药品分析中的数据处理和批量对批量比较.
根据分子量,重复单位,寡合体长度和末端组效应,阐明PEGs的电离趋势.

主要方法:

利用超临界流体染色学 (SFC) 进行PEG寡合物的先进分离.
采用质谱法 (MS) 采用选择性电离技术对PEG作为氨化分子.
开发了视觉表示来分析相对于聚合物分子量和重复单位的电离反应.

主要成果:

实现了聚乙烯糖醇 (PEG) 作为氨酸添加物的选择性电离,简化了质谱.
证明SFC-MS有效地分离PEG寡合体,使得分析更加清晰.
确定了影响电子喷雾电离的关键因素,包括寡合体长度和终端组修饰.

结论:

开发的选择性氨离子化的SFC-MS方法显著简化了复杂聚乙烯糖醇 (PEG) 的分析.
这种方法增强了数据处理,并促进了可靠的批量对批量比较,用于制药质量控制.
这些发现提供了一种强大的方法来表征PEGs,这对于优化药物输送系统至关重要.

关键词:

电子喷雾电离化电离化质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量聚乙烯糖醇 (PEG) 是一种聚乙烯基醇.聚合物是一种聚合物.超临界流体色谱学超临界流体色谱学

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