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在Dendrobium catenatum生长和发育期间对WOX家族基因的功能分析

Kai Chen¹, Hao Wang¹, Yi-Ting Chen¹

¹Nurturing Station for the State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Lin'an 311300, China.

Yi chuan = Hereditas

|August 23, 2023

概括

在Dendrobium catenatum中,与WUSCHEL相关的Homeobox (WOX) 基因调节植物发育. 在阿拉比多普西斯中DcWOX4,DcWOX9和DcWOX11的过度表达影响了叶子形状,开花时间和生育能力,揭示了保存和特定的角色.

科学领域:

植物分子生物学植物分子生物学
发展遗传学发展遗传学
兰花基因组学兰花基因组学

背景情况:

与WUSCHEL相关的Homeobox (WOX) 基因是植物特有的转录因子,对发育至关重要.
Dendrobium catenatum是一种罕见的中国草药,具有独特的生长特征.
了解DcWOX基因功能可以阐明兰花中保存和特定的发育途径.

研究的目的:

分析Dendrobium catenatum WOX (DcWOX) 基因的系系,时空表达和异质功能.
研究DcWOX基因在植物形态发生,叶子发育,开花和生育中的作用.

主要方法:

对DcWOX基因家族成员的家族遗传学分析.
对D. catenatum. 的时空表达模式的分析.
在转基因Arabidopsis thaliana中DcWOX4,DcWOX9和DcWOX11的异构表达.

主要成果:

DcWOX基因形成了三个不同的进化分支,具有多样化的组织表达.
在Arabidopsis中过度表达DcWOX4,DcWOX9和DcWOX11导致化,叶子形态变化 (针状,状,卷曲),花期延迟,生育能力降低.
异常与TCP,CUC,KNOX,FT,SOC1和CO基因的改变表达有关.

结论:

Dendrobium catenatum WOX基因在调节植物形态发生,叶子发育,开花时间和生育能力方面发挥着重要作用.
这些发现扩大了WOX基因家族已知的功能.
这项研究提供了有关兰花进化过程中保存和特定的发育机制的见解.

关键词:

WOX基因家族的WOX基因家族.转基因的转基因生物

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